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Apple dropped 32-bit app support in macOS Catalina (10.15). The official ADT binary is 32-bit, so it on modern macOS. You must use Method 3.

Add the MGLTools installation directory to your system's PATH. This allows you to run scripts like prepare_receptor4.py directly from any terminal. 3. Making It Work: Common Fixes

Always ensure your protein and ligand files are in the same working directory to avoid "File Not Found" errors during the simulation. Conclusion

ADT is just the GUI. To actually run docking, download:

If you need the latest AutoDock-GPU or AutoDock Vina, you may need to download those separately, but ADT remains the standard preparation tool.

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